Streptococcus Flow Chart
Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus bovis, Viridans group Streptococcus, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus
The tests including results that you will need to include are: Gram stain, catalase, hemolysis, optochin sensitivity/resistance, bacitracin sensitivity/resistance, bile solubility, CAMP test, coagulase test, 6.5% NaCl, bile esculin. Find results for each organism within your lab manual as well as using your own research skills. Keep in mind that not all tests are necessary for each organism and may not be relevant.
Start big picture with gram stain and cell morphology, then go into catalase for example. Use Microsoft word, Adobe, or PowerPoint to generate your chart. It should look similar to the flow chart on page 22 of your lab manual, but with your own creative flare and slightly more detail. I suggest using landscape instead of portrait mode. You will want to include the result for each test in addition to an explanation of what +/- result means and what it looks like. For example, bile esculin ++ means “survival on bile, hydrolysis of esculin, growth, black precipitate.” You will also want to include a key for those assays that contain an acronym, for example MSA on the chart should be listed as MSA: mannitol salt agar in the key. Further, limit your how chart to 1 page.
If you cannot fit the key on the front, you may include it on a separate page.
Formatting Guidelines: 1. Limit to one page (key can be on second page if no room) 2. Must include all tests and all organisms listed above 3. Each test should include the result (+/-), description (how it looks), and meaning (enzyme produced, hemolytic pattern, etc.) for the organism (some may have more than one) 4. Organization should be neat and easy to follow 5. Organisms should be italicized with the first initial capitalized and the species in lowercase 6. Each test should be listed in a logical manner with a single branch ultimately leading to a single organism near the bottom of the page. 7. Only necessary tests are listed for necessary organisms. For example, CAMP would not make sense to test with S. aureus.